Coding

Part:BBa_K3428002:Design

Designed by: Alexis Choi   Group: iGEM20_Stony_Brook   (2020-10-26)


TetR-UVR8 fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 736
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 70
    Illegal SapI site found at 751
    Illegal SapI.rc site found at 466


Design Notes

Since a negative control for this fusion protein was desired, a TetR repressor domain was employed so that under exposure of the chassis to tetracycline, the activator was prevented from being functional. The composition and flexibility of the linker was also considered to improve overall flexibility.

Note that this part did not undergo real-world characterization due to COVID-19 restrictions.

Source

BBa_K3428000, BBa_K416001

References

[1] Müller, K., Engesser, R., Schulz, S., Steinberg, T., Tomakidi, P., Weber, C. C., Ulm, R., Timmer, J., Zurbriggen, M. D., & Weber, W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic acids research, 41(12), e124. https://doi.org/10.1093/nar/gkt340

[2] Wu, M., Eriksson, L.A. & Strid, Å. Theoretical prediction of the protein–protein interaction between Arabidopsis thaliana COP1 and UVR8. Theor Chem Acc 132, 1371 (2013). https://doi.org/10.1007/s00214-013-1371-7

[3] Yang, X., Montano, S., & Ren, Z. (2015). How Does Photoreceptor UVR8 Perceive a UV-B Signal?. Photochemistry and photobiology, 91(5), 993–1003. https://doi.org/10.1111/php.12470